Recognition of chemical carcinogen-modified DNA by a DNA-binding protein.
نویسندگان
چکیده
منابع مشابه
Carcinogen-modified DNA and specific humoral immunity toward carcinogen-DNA adducts. A review.
Carcinogenesis is a complex and multistep process starting from initiation to tumor progression. Damage to DNA, induced by the covalent binding of chemical carcinogens on critical DNA segments, reflects exposure and is directly related to tumor formation. For this reason it's very important detect and quantify DNA-adducts by using highly sensitive methods. During the last 30 years sophisticated...
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One obstacle to achieving complete understanding of the principles underlying sequence-dependent recognition of DNA is the paucity of structural data for DNA recognition sequences in their free (unbound) state. Here, we carried out crystallization screening of 50 DNA duplexes containing cognate protein binding sites and obtained new crystal structures of free DNA binding sites for three distinc...
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Benzo[alpha]pyrene diol epoxide (BPDE) is a well-studied environmental carcinogen that binds covalently to DNA. Here we describe a photochemical technique that allows us to map BPDE-binding sites within cloned gene sequences. The technique is based upon our observation that, when irradiated with laser light at 355 nm, one single-strand DNA cut is produced at each BPDE binding site. In initial e...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.6.3201